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Absorption and Fluorescence Spectra of Safranin O (aka., Basic Red #2)

A project log for DAV5 V2.1 Spectrometer

The ZEN of simplicity Making the Complex...Seem Simple

david-h-haffner-srDavid H Haffner Sr 07/20/2016 at 13:440 Comments

http://publiclab.org/notes/dhaffnersr/07-20-2016/absorption-and-fluorescence-spectra-of-safranin-o-aka-basic-red-2

This is a fundamental tutorial using the Red dye Safranin O, to illustrate the steps for preforming absorption spectra, irregardless of whatever data processing software you are using or not.

I am using the Public Lab v2.5 Spectrometer to demonstrate these steps. The processing and capture software I used was Spekwin32 v172.2p5 proto type, although I know that Jeff @Warren and Dave @Stoft are working diligently to incorporate this feature into Spectral Workbench. I'm no graphic artist, so please bear with this presentation, I have made every effort to be as accurate as possible, but there can still be a margin of error.

I am presenting the basic set up here and will provide the link to the entire research note:

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