Things to do list!

A project log for Plastic-producing personal bioreactor system

Cultivate a [safe!] bacteria that accumulates PHA, then recover the PHA granules, purify, and use for 3d-print filament!

Neil K. SheridanNeil K. Sheridan 03/31/2019 at 21:350 Comments

1. Obtain 1kg of Polyhydroxyalkaonate granules is ~£215 from sigma aldrich , then test if these can be made into 3d-printing filament using e.g. an extruder like the filabot.

2. If ok with making filament, then try some 3d prints! e.g. just a water holder for drinking!


3. Obtain Cupriavidus necator culture (e.g. 185,- €) and experiment with the growth and PHA accumulation phases using protocols from papers mentioned earlier. You will need to invest in various analytic tools!


Q. What should Ralstonia eutropha be fed with?

Q. Is wild-type of an engineered type best?

Q. Which PHA is the best for 3d-printing? Depending on what we are printing of course!

A. [1] describes PHAs as 

PropertiesExtrusion TempProsCons
Several copolymers,
brittle and stiff
~160CUV-stable, stiffnessElasticity, brittle

So I'm not sure, since there are such a wide variety in the family! 

Two of the common members of the PHA family I have encountered so far:

- Poly(3-hydroxybutyrate) aka P(3HB)

- P(3HB-co-3HHx)


1. Pakkanen, J., Manfredi, D., Minetola, P., & Iuliano, L. (2017). About the Use of Recycled or Biodegradable Filaments for Sustainability of 3D Printing. Smart Innovation, Systems and Technologies, 776–785.doi:10.1007/978-3-319-57078-5_73