1. Obtain 1kg of Polyhydroxyalkaonate granules is ~£215 from sigma aldrich , then test if these can be made into 3d-printing filament using e.g. an extruder like the filabot.
2. If ok with making filament, then try some 3d prints! e.g. just a water holder for drinking!
CHECKPOINT PASSED! [yes/no]
3. Obtain Cupriavidus necator culture (e.g. 185,- €) and experiment with the growth and PHA accumulation phases using protocols from papers mentioned earlier. You will need to invest in various analytic tools!
Q. What should Ralstonia eutropha be fed with?
Q. Is wild-type of an engineered type best?
Q. Which PHA is the best for 3d-printing? Depending on what we are printing of course!
A.  describes PHAs as
brittle and stiff
|~160C||UV-stable, stiffness||Elasticity, brittle|
So I'm not sure, since there are such a wide variety in the family!
Two of the common members of the PHA family I have encountered so far:
- Poly(3-hydroxybutyrate) aka P(3HB)
1. Pakkanen, J., Manfredi, D., Minetola, P., & Iuliano, L. (2017). About the Use of Recycled or Biodegradable Filaments for Sustainability of 3D Printing. Smart Innovation, Systems and Technologies, 776–785.doi:10.1007/978-3-319-57078-5_73