Project log #9 "It's all comin together"

A project log for Home Biology Lab

A diy bio lab for mammalian cell culture, genetic engineering, and whatever creations cross my mind

saporanoSapoRano 08/22/2023 at 05:390 Comments


So I finally have almost all the hardware to grow E.coli and make them competent

I'm using the frozen pea technique for the ice bath  

(a box full of frozen peas)

And I've been looking into a way to make a heat block for a while

I was about to make one and have a friend with a drill press make the holes into the aluminium and just go through the trouble of making it myself 

But I found a working heat block with free shipping and a return policy on ebay for $35!

If it works that will be everything I need for the growing and competency parts but I still need an autoclave and a air filter of some kind

Now the autoclave is simple just a pressure cooker off amazon 50 bucks done deal 

But the air filter is a bit for trouble 

I've been thinking of a design that would work and this is the plan currently

Using two merv 13 filter and two carbon filtration sheets sandwiched between (linked below)

With a uvc bulb in the future should be all I need 

From their all we do is take any fan any box throw it all together with some airtight silicone and mounts and diy easy air filter

I might film a video of me making and put it on the channel but I probably won't  

The Stanford Freegenes group haven't gotten back to me yet but the hope is still alive until they say no I guess.

Still working on the youtube channel hopefully the next video will actually be intresting 

I'm going to be rating incubators on a tier list based on how good they are for DIY bio

Sounds fun but I still need to record the audio and edit, I finished the script though

I'm still trying to sell as much as I can because I really need the money 

Speaking of money go to my patreon I really need it 😭

Also if you have any ideas or advice message me or comment them, all press is good press at the end of the day

It's 1AM and I'm tired