The 3D model sketching step is underway. I contacted for the luer lock specifications but got no response so I stopped waiting and assumed that the luer lock used was the ISO 80369-7 standard .

I carried out the plan in model 4 first, there are two plans for this type, the first is to utilize the tube with luer lock to circulate CO2 and O2, the second plan is to design a separate air circulation part. The picture below is plan 1:

Option 2 also has some different shaped air circulation unit options for sample study:

All options choose the side opening gate because this structure will help the 3D printing more stable and I don't want to print many support structures, which means saving material.