How it Works - Back-Scattering Configuration
(Green = 532 nm excitation laser, yellow/red = Raman scattering)

The 532 nm laser fires horizontally. The bandpass filter strips IR leakage from the cheap diode module and narrows the laser line. The beam hits the DMLP550 dichroic mirror at 45°, which folds it 90° downward through the 20x infinity-corrected objective and onto the sample.
Back-scattered light travels back up through the objective. The Raman-shifted photons (>550 nm) transmit straight through the dichroic toward the detector, while the unwanted Rayleigh-scattered 532 nm light continues into the beam dump. The FELH0550 longpass filter provides a second stage of Rayleigh rejection, the f = 19 mm achromat focuses the collimated beam onto the 100 µm slit, and the B&W Tek spectrometer records the spectrum.
Signal Processing

The acquired spectrum is processed to remove residual background and fluorescence and make it legible: cropping and Raman-shift conversion, cosmic spike removal, baseline correction (ALS / arPLS / SNIP), smoothing and normalization. Peaks can additionally be detected, fitted (pseudo-Voigt) and matched against a reference library - though fitting matters more for high-resolution, calibrated instruments than for this one, where the 100 µm slit sets the resolution floor.
The processing GUI (CubeRaman-SpectrumPro) is included in the repo.
Performance & Sample Results
Verified (and pictured): polypropylene/-ethylene, paracetamol, isopropyl alcohol, ethanol, diamond, beta-carotene and more. Depending on focus depth / working distance, the instrument can even measure liquids through thin container walls - the objective's (here: 2.4 mm) working distance decides what's reachable.
Status & Next Steps
Ongoing. Current limitations are the 100 µm slit (resolution bottleneck), residual stray light, and magnet-held kinematics that aren't yet truly preloaded. The next iteration will be more compact and rugged - CNC-milled from aluminum.
Recent Updates - YouTube Video
Build Process - YouTube Video
Jacob

David H Haffner Sr
esben rossel
wnodvik