PCR machines copy DNA. The CCPCR uses a coffee cup, an Arduino and a few other items.Cost to build is about 25$.
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This idea was inspired by Russell Durrett's Light Bulb PCR Machine. http://russelldurrett.com/lightbulbpcr.html
I decided to attempt to make a similar device with parts I already had on hand. I replaced the light bulb with a 3D printing heating element from an old 3D printer. The 40mm fan came from the printer as well. The UNO, temperature sensor and relay came from an Arduino starter kit I had. I did have to buy the 12VDC/5 Amp power supply since the 12VDC/3Amp one I had was insufficient.
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Zip Archive - 13.91 kB - 10/16/2017 at 00:49 |
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CCPCRv1.zipArduino CodeZip Archive - 3.69 kB - 10/09/2017 at 14:55 |
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The Arduino code uses the serial monitor to take the following input.
Denaturation Stage Temperature (C)
Denaturation Stage Duration (sec)
Annealing Stage Temperature (C)
Annealing Stage Duration (sec)
Extension Stage Temperature (C)
Extension Stage Duration (sec)
Number of Cycles
Once these values re entered the process begins. The system is first raised to the Denaturation Stage temperature and held at this temperature for 120 seconds. The the Denaturation, Annealing and Extension stages are executed and repeated for the number of cycles entered.
The current code, CCPCRv1, runs the following fixed routine.
0. Raise temp to 95C
1. Stay at 95C for 120 seconds
2. Stay at 95C for 15 seconds
3. Drop temp to 55C
4. Stay at 55C for 15 seconds
5. Raise temp to 68C
6. Stay at 68C for 45 seconds
7. Raise temp to 95C
*8. Repeat steps 2 through 17 nineteen times. *(Not Implemented yet)
9. End
Use the Arduino IDE serial port monitor to watch progress.
Hot glue the fan to the plywood top directly over the center hole.
Then wire the components based on the sketch below.
1. Cut a small 1/4" piece plywood into a 4 1/2" Square
2. Cut holes as shown in sketch
Paul McCabe
ProtoVoltaics
josean.brasero
Parker Newman
I stuck a link to your PCR build in my 1 week old unheard of youtube channel if thats OK
https://www.youtube.com/watch?v=52A_F66Q0d8