A DIY Nanopore DNA sequencer

Nanopore membranes are low cost. What about a DIY DNA sequencer based on a Reverse Osmosis water filter?

Public Chat
Similar projects worth following
There were several generations of sequencers, and the driving idea is to cut the DNA in very very short pieces that could be analyzed separately and accurately.
However the tiny reads have to be reassembled and it is a hard challenge. So much for all the pop science articles.

There are sequencers that are able to process long reads. Not surprisingly their error rate is awful.

Oxford Nanopore smallest sequencer (as big as a USB dongle) is still valuable with lot of data processing. But a one shot usage of their device costs near $800 and it is slooooow

Their core idea is to make a DNA molecule go through a nanometer pore, and to measure how the current flow is varying as each nucleotide goes through the pore.

Basically their device is very similar to a water reverse osmosis unit with an electrical potential applied to the water chamber.
The real added value of Oxford Nanopore is in the software, not in the hardware.

Enjoy this project?



历史精神 wrote 02/13/2020 at 08:37 point

Not much information on how to replicate a nanopore that can sense the pass-through of DNA. At least,you need a  smolecular channel that has appropriate pore size which can genearte agitated voltage when DNA is pulled through.  There should also be a DNA helicase to unwind the dsDNA. 

  Are you sure? yes | no

Similar Projects

Does this project spark your interest?

Become a member to follow this project and never miss any updates