Quantity   Component name
1 × Raspberry Pi 2 or 3
1 × 16 GB mini SD card card is installed with raspian
1 × HDMI to VGA adapter
1 × Monitor
1 × Mouse (USB)
1 × Keyboard (USB) Development Kits, Boards and Systems / Development Kits and Boards
1 × Arduino nano ATmega328 Controls all periohericals except camera
1 × Custom printed circuit board (PCB) makes the connection between Arduino and periphericals easier
1 × 3D printed parts Mainframe and holders for camera, led ring and etc.
1 × Adjustable focus RPi Camera http://www.wvshare.com/product/RPi-Camera-B.htm
1 × USB A to mini B cable connects arduino Nano to the raspberry pi
1 × RGB LED (marker for peltier)
3 × 220 OHM resistors For RGB LEDs
1 × Paper sheet Diffuse light to evenly illuminate samples.
1 × Adafruit 8X8 blue LED matrix https://www.adafruit.com/products/1052
1 × Adafruit 12 LED ring https://www.adafruit.com/products/1643
1 × 100 nF capacitor filter for the LED ring (together with resistor below)
1 × 150 Ohm resistor filter for LED ring
1 × AD22100 Temperature sensor measures temperature in the peltier element
1 × 1k Ohm resistor filter for temperature sensor
1 × 100nF capacitor filter for temperature sensor
1 × Peltier element TEC1 127 05 (40X40X4mm)
1 × Heat sink for Peltier (35x35x20MM)
1 × Fan for Peltier (40X40X10mm 5V)
1 × H-brigde L298N https://www.sparkfun.com/products/9479
1 × heat sink for H-bridge right now, 40X40X10, but this maybe an overshoot
1 × Resistor for H-bridge ( 1 Ohm able to take 20W) Current sensing resistor for L298N
2 × Capacitors for H-bridge (100nF)
1 × Strong Blue LED (3W) 410 nm For optogenetic stimulation
1 × Heat sink for strong blue LED (35X35X10mm)
2 × Resistor for blue LED (15 Ohm able to take 3W) For limiting the high-power LED current
1 × Excitation filter (cheap plastic foils used in theater lighting) This prevents unwanted light wavelenghts from reaching the sample to be imaged
1 × Emission filter (cheap plastic foils used in theater lighting) This prevents unwanted light wavelenghts from reaching the camera
1 × M3 headcap screws (~20-30mm) used to fix raspberry pi, arduino holder and other parts to the printed parts
1 × Thermal paste for contacts in between thermistor and peltier. Also contacts with heatsinks
3 × Switching voltage regulator LM 2596 Regulators used to convert 12V from the power source to 5V. The three lines will power the pi, high power ports and LED ring and matrix.
3 × 33µh inductor Used in the power circuit
3 × Capacitor 680µF 16V Used in power circuit
3 × Capacitor 220µF 16V Used in power circuit
3 × DIODE SCHOTTKY 5A 30V - 1N5824 Used in power circuit
1 × Barrel jack connector 2.1mm positive center
1 × 12V 5A power brick 2.1 positive center plug
1 × A to micro B USB connection in between PCB and raspberry Pi
9 × 2 pin screw terminal connector 3.5mm connection between PCB and peripherals
3 × 3 pin screw terminal connector 3.5mm connection between PCB and peripherals
3 × BC268 transistor NPN transistor BEC
3 × 270 Ohm resisors in between transistor base and control signal