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Absorption Spectra of Methylene Blue in Water

A project log for DAV5 V3.01 Raman Spectrometer

The only thing worth doing, is the thing worth doing right!

david-h-haffner-srDavid H Haffner Sr 01/28/2017 at 15:013 Comments

I tested the current absorption capabilities of this spectrometer and its resolution and I am definitely pleased with the results so far. There is still a lot of tweaking and adjustments to be made but those issues will be mostly resolved when I get the new 1800 ln/mm diffractive grating (Holographic) and the Raman edge filter.

This is a great start though, these plots represent a comparison between my spectrometer and the SpexFluoroMax ll used by the Oregon Medical Laser Center back in 1995-96. My sample of Methylene blue is the same as the one they used (concentration of 10ul) in a quartz cuvette in distilled water.

Although their light source is superior to mine because of its stability, my Solux 50W 4700k isn't too bad (using a regulated 12vdc 5A power supply)

Discussions

Thomas wrote 01/28/2017 at 15:25 point

Nice results! Congrats!

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David H Haffner Sr wrote 01/28/2017 at 15:33 point

Thanks Thomas, I know it looks a little ugly right now but it'll get better :)

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Thomas wrote 01/28/2017 at 15:39 point

Dedication solves problems, and you appear to have plenty of it!

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