1. Obtain 1kg of Polyhydroxyalkaonate granules is ~£215 from sigma aldrich , then test if these can be made into 3d-printing filament using e.g. an extruder like the filabot.
2. If ok with making filament, then try some 3d prints! e.g. just a water holder for drinking!
CHECKPOINT PASSED! [yes/no]
3. Obtain Cupriavidus necator culture (e.g. 185,- €) and experiment with the growth and PHA accumulation phases using protocols from papers mentioned earlier. You will need to invest in various analytic tools!
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Q. What should Ralstonia eutropha be fed with?
Q. Is wild-type of an engineered type best?
Q. Which PHA is the best for 3d-printing? Depending on what we are printing of course!
A. [1] describes PHAs as
Properties | Extrusion Temp | Pros | Cons |
Several
copolymers,
brittle and stiff | ~160C | UV-stable, stiffness | Elasticity, brittle |
So I'm not sure, since there are such a wide variety in the family!
Two of the common members of the PHA family I have encountered so far:
- Poly(3-hydroxybutyrate) aka P(3HB)
- P(3HB-co-3HHx)
References:
1. Pakkanen, J., Manfredi, D., Minetola, P., & Iuliano, L. (2017). About the Use of Recycled or Biodegradable Filaments for Sustainability of 3D Printing. Smart Innovation, Systems and Technologies, 776–785.doi:10.1007/978-3-319-57078-5_73
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